Plant Transcription Factor Database
Previous version: v3.0
Tarenaya hassleriana
HSF Family
Species TF ID Description
XP_010518819.1HSF family protein
XP_010521560.1HSF family protein
XP_010523506.1HSF family protein
XP_010525224.1HSF family protein
XP_010526389.1HSF family protein
XP_010526390.1HSF family protein
XP_010526391.1HSF family protein
XP_010526392.1HSF family protein
XP_010527205.1HSF family protein
XP_010527455.1HSF family protein
XP_010529215.1HSF family protein
XP_010530018.1HSF family protein
XP_010530513.1HSF family protein
XP_010531239.1HSF family protein
XP_010532738.1HSF family protein
XP_010532852.1HSF family protein
XP_010532853.1HSF family protein
XP_010538384.1HSF family protein
XP_010538799.1HSF family protein
XP_010540690.1HSF family protein
XP_010540760.1HSF family protein
XP_010540761.1HSF family protein
XP_010541155.1HSF family protein
XP_010543464.1HSF family protein
XP_010545101.1HSF family protein
XP_010545102.1HSF family protein
XP_010546501.1HSF family protein
XP_010547515.1HSF family protein
XP_010549838.1HSF family protein
XP_010549839.1HSF family protein
XP_010550365.1HSF family protein
XP_010554824.1HSF family protein
XP_010554825.1HSF family protein
XP_010556232.1HSF family protein
XP_010556233.1HSF family protein
XP_010556876.1HSF family protein
XP_010556877.1HSF family protein
XP_010558210.1HSF family protein
XP_010558628.1HSF family protein
XP_010558629.1HSF family protein
HSF Family Introduction

Heat stress transcription factors (Hsfs) are the major regulators of the plant heat stress (hs) response. Sequencing of the Arabidopsis genome revealed the existence of 21 open-reading frames (ORFs) encoding putative Hsfs assigned to classes A-C. Here we present results of a functional genomics approach to the Arabidopsis Hsf family focused on the analysis of their C-terminal domains (CTDs) harboring conserved modules for their function as transcription factors and their intracellular localization. Using reporter assays in tobacco protoplasts and yeast as well as glutathione-S-transferase (GST) pull-down assays, we demonstrate that short peptide motifs enriched with aromatic and large hydrophobic amino acid (aa) residues embedded in an acidic surrounding (AHA motifs) are essential for transcriptional activity of class A Hsfs. In contrast to this, class B and C Hsfs lack AHA motifs and have no activator function on their own. We also provide evidence for the function of a leucine (Leu)-rich region centered around a conserved QMGΦL motif at the very C-terminus as a nuclear export signal (NES) of class A Hsfs. Sequence comparison indicates that the combination of a C-terminal AHA motif with the consensus sequence FWxxF/L,F/I/L as well as the adjacent NES represents a signature domain for plant class A Hsfs, which allowed to identify more than 60 new Hsfs from the expressed sequence tag (EST) database.

Kotak S, Port M, Ganguli A, Bicker F, von Koskull-Doring P.
Characterization of C-terminal domains of Arabidopsis heat stress transcription factors (Hsfs) and identification of a new signature combination of plant class A Hsfs with AHA and NES motifs essential for activator function and intracellular localization.
Plant J, 2004. 39(1): p. 98-112.
PMID: 15200645